Hyperphosphorylation of the asialoglycoprotein receptor in isolated rat hepatocytes following ethanol administration
نویسندگان
چکیده
منابع مشابه
Hyperphosphorylation of the asialoglycoprotein receptor in isolated rat hepatocytes following ethanol administration.
Ethanol administration leads to altered function and impaired receptor-mediated endocytosis of the hepatocyte asialoglycoprotein receptor (ASGP-R). The purpose of the present study was to examine the effects of ethanol on the phosphorylation of the ASGP-R to determine whether this post-translational modification could contribute mechanistically to the observed ethanol-induced alterations. The m...
متن کاملRecycling of the Hepatic Asialoglycoprotein Receptor in Isolated Rat Hepatocytes
We recently reported that the dissociation of internalized receptor-1261-asialo-orosomucoid (ASOR) complexes by isolated hepatocytes is a biphasic process; most complexes dissociate rapidly but 25-50% dissociate slowly (Oka, J. A., and Weigel, P. H. J. Biol. Chern. 258, 10253-10262). Cells were allowed to endocytose a pulse of surface-bound '''I-ASOR, and were washed and then incubated at 37 "C...
متن کاملLactate-stimulated ethanol oxidation in isolated rat hepatocytes.
1. Hepatocytes isolated from starved rats and incubated without other substrates oxidized ethanol at a rate of 0.8-0.9mumol/min per g wet wt. of cells. Addition of 10mm-lactate increased this rate 2-fold. 2. Quinolinate (5mm) or tryptophan (1mm) decreased the rate of gluconeogenesis with 10mm-lactate and 8mm-ethanol from 0.39 to 0.04-0.08mumol/min per g wet wt. of cells, but rates of ethanol ox...
متن کاملExpression of the asialoglycoprotein receptor in cultured rat hepatocytes is modulated by cell density.
The influence of cell density on expression of the asialoglycoprotein receptor system in primary cultures of rat hepatocytes was evaluated by measuring the level of the receptor specific mRNA. When the hepatocytes are cultured at high cellular density and are not in a proliferative condition, the transcript molecules of the receptor appear increased about 50% with respect to the low plating den...
متن کاملMetabolism and Cytotoxic Mechanisms of Nitroglycerin in Isolated Rat Hepatocytes
It has been proposed that organic nitrates such as glyceryl trinitrate (GTN), used in the treatment of cardiovascular diseases, act by producing nitric oxide (NO). However, the biochemical pathway for NO formation from GTN is not well understood. In the present study, we showed that nitrate formation from GTN, by isolated rat hepatocytes, was inhibited about 50% when cellular glutathione w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Pharmacology
سال: 2000
ISSN: 0006-2952
DOI: 10.1016/s0006-2952(00)00353-1